Protein Isolation from the Developing Embryonic Mouse Heart Valve Region

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Biology

JoVE Journal
Biology

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JoVE Journal Biology

Protein Isolation from the Developing Embryonic Mouse Heart Valve Region

DOI:

10.3791/51911-v

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06:55 min

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September 23, 2014

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Laura A. Dyer, Yaxu Wu, Cam Patterson

¹McAllister Heart Institute,University of North Carolina at Chapel Hill, ²New York-Presbyterian Hospital/Weill-Cornell Medical Center

Chapters

00:05Title
01:00Aortic Valve Excision and Protein Extraction
04:53Results: Representative Embryonic Aortic Valve Analyses
06:24Conclusion

Summary

Automatic Translation

The analysis of protein expression in young embryonic mouse valves has been hampered by the limited tissue available. This manuscript provides a protocol for preparing protein from developing embryonic mouse valve regions for western blot analysis.

Protein Isolation from the Developing Embryonic Mouse Heart Valve Region

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