The analysis of protein expression in young embryonic mouse valves has been hampered by the limited tissue available. This manuscript provides a protocol for preparing protein from developing embryonic mouse valve regions for western blot analysis.
Dyer, L. A., Wu, Y., Patterson, C. Protein Isolation from the Developing Embryonic Mouse Heart Valve Region. J. Vis. Exp. (91), e51911, doi:10.3791/51911 (2014).