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Immunology and Infection

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JoVE Journal Immunology and Infection
Real-time Imaging of Myeloid Cells Dynamics in ApcMin/+ Intestinal Tumors by Spinning Disk Confocal Microscopy
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Real-time Imaging of Myeloid Cells Dynamics in ApcMin/+ Intestinal Tumors by Spinning Disk Confocal Microscopy

Article DOI: 10.3791/51916 • 05:21 min • October 6th, 2014
October 6th, 2014 •
*1,2, *2, 2
1Department of Oncology, INSERM U661, Functional Genomic Institute, 2Department of Anatomy, University of California
* These authors contributed equally

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By using transgenic reporter mice and injectable fluorescent labels, long-term intravital spinning disk confocal microscopy enables direct visualization of myeloid cell behavior into intestinal adenoma in the ApcMin/+ colorectal cancer model.

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Real-time Imaging Myeloid Cells Dynamics ApcMin/+ Intestinal Tumors Spinning Disk Confocal Microscopy Intravital Imaging Techniques Live Cellular Behavior Organ And Tumor Accessibility Intestine Surgical Procedure Transgenic Fluorescent Reporter Mice Injectable Tracers Or Antibodies Four-color Multi-region Micro-lensed Spinning Disk Confocal Microscope Long-term Continuous Imaging Rapid Image Acquisition ApcMin/+ Mice Multiple Adenomas Small Intestine C-fms-EGFP Mice ACTB-ECFP Mice Intestinal Epithelial Cells Crypts Labeling Tumor Components Blood Vessels Neutrophils Positioning The Tumor For Imaging Serosal Surface Time-lapse Movies
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