From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia

Benedetta Apollonio; Maria Teresa Sabrina Bertilaccio; Umberto Restuccia; Pamela Ranghetti; Federica Barbaglio; Paolo Ghia; Federico Caligaris-Cappio; Cristina Scielzo

doi:10.3791/51942

Journal

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Medicine

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From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia

JoVE Journal
Medicine

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JoVE Journal Medicine

From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia

13,713 Views

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10:18 min

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October 19, 2014

DOI:

10.3791/51942-v

DOI:

10.3791/51942-v

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10:18 min

October 19, 2014

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13689 Views

Benedetta Apollonio^1,2, Maria Teresa Sabrina Bertilaccio¹, Umberto Restuccia³, Pamela Ranghetti¹, Federica Barbaglio¹, Paolo Ghia^1,4, Federico Caligaris-Cappio^1,4, Cristina Scielzo¹

¹Division of Molecular Oncology,IRCCS, San Raffaele Scientific Institute ²Department of Haemato-Oncology,King’s College London ³IFOM, FIRC Institute of Molecular Oncology ⁴Università Vita-Salute San Raffaele

Summary

Here we describe a protocol that couples two proteomic techniques, namely 2-dimensional Electrophoresis (2DE) and Mass Spectrometry (MS), to identify differentially expressed/post-translational modified proteins among two or more groups of primary samples. This approach, together with functional experiments, allows the identification and characterization of prognostic markers/therapeutic targets.