Here we describe a protocol that couples two proteomic techniques, namely 2-dimensional Electrophoresis (2DE) and Mass Spectrometry (MS), to identify differentially expressed/post-translational modified proteins among two or more groups of primary samples. This approach, together with functional experiments, allows the identification and characterization of prognostic markers/therapeutic targets.
Apollonio, B., Bertilaccio, M. T. S., Restuccia, U., Ranghetti, P., Barbaglio, F., Ghia, P., Caligaris-Cappio, F., Scielzo, C. From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia. J. Vis. Exp. (92), e51942, doi:10.3791/51942 (2014).