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RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord
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JoVE Journal Neuroscience
RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord

RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord

DOI:
10.3791/51951-v

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11:13 min

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November 01, 2014

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1Department of Cell and Developmental Biology,Universidade de São Paulo

Chapters

  • 00:05Title
  • 01:22Harvesting Neural Tubes for RNA Collection
  • 04:32Pooling Tissue to Purify RNA for Illumina Sequencing
  • 05:34Comparing RNA-Seq Datasets on the Galaxy Public Server
  • 09:52Results: Transcriptome Analyis Quality
  • 10:42Conclusion

Summary

Automatic Translation

Automatic Translation

This video shows the basic steps for performing whole transcriptome analysis on dissected chick embryonic spinal cord samples after transfection with in ovo electroporation.

Tags

Keywords: RNA-SeqDifferential Gene ExpressionChick Embryonic Spinal CordIn Ovo ElectroporationNeural DevelopmentTranscriptome AnalysisGene FunctionBioinformaticsGalaxy Public ServerHigh-quality RNA SamplesOverexpression

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