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JoVE Journal
Immunology and Infection

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Propagation of Homalodisca coagulata virus-01 via Homalodisca vitripennis Cell Culture
 

Propagation of Homalodisca coagulata virus-01 via Homalodisca vitripennis Cell Culture

Article DOI: 10.3791/51953
September 25th, 2014

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Summary September 25th, 2014

Here we present a protocol to propagate Homalodisca vitripennis cells and HoCV-1 in vitro. Medium was removed from HoCV-1 positive cultures and RNA extracted every 24 hr for 168 hr. Cell survivability was quantified by trypan blue staining. Whole virus particles were extracted post-infection. Extracted RNA was quantified by qRT-PCR.

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