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JoVE Journal
Neuroscience

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Vibratome Sectioning Mouse Retina to Prepare Photoreceptor Cultures
 

Vibratome Sectioning Mouse Retina to Prepare Photoreceptor Cultures

Article DOI: 10.3791/51954
December 22nd, 2014

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Summary December 22nd, 2014

Neural retina of a mouse aged 8 days is on top of a 4% gelatin block. After isolation of the photoreceptor layer (200 µm) by vibratome, the photoreceptors are seeded after mechanical and enzymatic dissociation for culture. The photoreceptor layer can be used for molecular, biochemical analyses or transplantation.

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