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JoVE Journal Biology
In vitro Methylation Assay to Study Protein Arginine Methylation
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In vitro Methylation Assay to Study Protein Arginine Methylation

Article DOI: 10.3791/51997
October 5th, 2014 •
1, 1, 1, 1, 1, 1,2, 1,3
1Department of Pulmonary, Critical Care, Sleep, and Allergy, University of Illinois at Chicago, 2Department of Hematology and Oncology, University of Illinois at Chicago, 3Jesse Brown Veterans Affairs Medical Center

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October 5th, 2014

Protein arginine methylation, catalyzed by a class of enzymes viz., protein arginine methyl transferases (PRMTs), is the process of enzymatic addition of methyl group(s) to arginines within proteins. The in vitro methylation assay is the most dependable tool for assessing the methylation status of known or novel PRMT substrates.

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