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In vitro Methylation Assay to Study Protein Arginine Methylation
<em>In vitro </em>Methylation Assay to Study Protein Arginine Methylation
JoVE Journal
Biology
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JoVE Journal Biology
In vitro Methylation Assay to Study Protein Arginine Methylation

In vitro Methylation Assay to Study Protein Arginine Methylation

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10:01 min

October 05, 2014

DOI:

10:01 min
October 05, 2014

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Summary

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Protein arginine methylation, catalyzed by a class of enzymes viz., protein arginine methyl transferases (PRMTs), is the process of enzymatic addition of methyl group(s) to arginines within proteins. The in vitro methylation assay is the most dependable tool for assessing the methylation status of known or novel PRMT substrates.

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