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In vitro Methylering Assay til at studere protein Arginin Methylering
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JoVE Journal Biology
In vitro Methylation Assay to Study Protein Arginine Methylation

In vitro Methylering Assay til at studere protein Arginin Methylering

DOI:
10.3791/51997-v

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10:01 min

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October 05, 2014

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, , , , , ,
1Department of Pulmonary, Critical Care, Sleep, and Allergy,University of Illinois at Chicago, 2Department of Hematology and Oncology,University of Illinois at Chicago, 3Jesse Brown Veterans Affairs Medical Center

Chapters

  • 00:05Title
  • 01:54Purification of Protein Arginine Methyl Transferases (PRMTs)
  • 05:31Methylation Assay
  • 07:16Confirmation of PRMT Expression and Equal Loading of Substrate
  • 08:38Results: PRMT1 Methylates Ras-GTPase Activating Protein Binding Protein 1 (G3BP1)
  • 09:40Conclusion

Summary

Automatic Translation

Automatic Translation

Protein arginin methylering, katalyseret af en klasse af enzymer, nemlig. Protein arginin metyltransferaser (PRMTs), er processen med enzymatisk tilsætning af methyl-gruppe (r) til argininer i proteiner. In vitro methylering analysen er den mest pålidelige redskab til at vurdere status for methylering af kendte eller nye PRMT substrater.

Tags

Protein Arginine MethylationPRMT1In Vitro Methylation AssayG3BP1S-adenosyl-L-[methyl-3H] MethioninePost-translational ModificationMethyl-arginine Specific AntibodiesProteomic ApproachesBiochemical Assays

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