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Chapters
- 00:05Title
- 01:01Preparation of Materials and Reagents
- 02:43Plating the Bottom Layer of Agar
- 04:20Plating the Upper Layer of Agar Containing Cells
- 06:19Staining the Upper Layer of Agar Containing Cells
- 06:47Results: The Soft Agar Colony Formation Assay
- 07:33Conclusion
The soft agar colony formation assay is a method used to confirm cellular anchorage-independent growth in vitro. The goal of this protocol is to illustrate a stringent method for the detection of the tumorigenic potential of transformed cells and the tumor suppressive effects of proteins on transformed cells.
