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The Analysis of Neurovascular Remodeling in Entorhino-hippocampal Organotypic Slice Cultures
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JoVE Journal Neuroscience
The Analysis of Neurovascular Remodeling in Entorhino-hippocampal Organotypic Slice Cultures

The Analysis of Neurovascular Remodeling in Entorhino-hippocampal Organotypic Slice Cultures

DOI:
10.3791/52023-v

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08:27 min

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October 23, 2014

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, ,
1Anatomical Institute, Department of Biomedicine Basel,University of Basel, 2Department of Neonatology, University Children’s Hospital (UKBB),University of Basel

Chapters

  • 00:05Title
  • 01:20Organotypic Entorhino-hippocampal Slice Cultures
  • 02:37In Vitro Hypoxia and Oxygen-glucose Deprivation (OGD) with Reperfusion
  • 04:28Fixation and Immunostaining
  • 05:47Determination of Regional Vessel Density
  • 06:22Results: Neuronal Death and Blood Vessel Loss after Hypoxia or OGD
  • 07:41Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol for entorhino-hippocampal organotypic slice cultures, which allows reproducing many aspects of ischemic brain injury, is presented. By studying changes of the neurovasculature in addition to changes in the neurons, this protocol is a versatile tool to study plastic changes in neural tissue after injury.

Tags

Brain IschemiaNeurovascular RemodelingOrganotypic Slice CulturesOxygen And Glucose DeprivationIn Vitro ModelNeuronal DamageVascular Changes

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