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Generation of Induced Pluripotent Stem Cells from Muscular Dystrophy Patients: Efficient Integration-free Reprogramming of Urine Derived Cells

Article DOI: 10.3791/52032 • 09:11 min • January 28th, 2015
January 28th, 2015 •
1, 1
1Department of Medicine, Medical College of Wisconsin

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This protocol entails detailed procedures for isolation of urine derived cells from muscular dystrophy patients; their efficient and rapid reprogramming through Sendai virus transduction.

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Induced Pluripotent Stem Cells Muscular Dystrophy Urine-derived Cells Reprogramming Dystrophic Cardiomyopathy In Vitro Disease Model Systems Drug Screening Studies Sendai Virus Vector Yamanaka Factors Fully Reprogrammed Clones Pluripotent Cells Vector-free Cardiomyocytes Disease Mechanisms Drug Screening
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