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Expression of Fluorescent Proteins in Branchiostoma lanceolatum by mRNA Injection into Unfertilized Oocytes
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1Département de Biologie du Développement et Cellules Souches,Institut Pasteur, 2Laboratoire de Biologie du Développement de Villefranche-sur-Mer (UMR7009 CNRS/UPMC Univ Paris 06),Sorbonne Universités, 3Equipe Epigenetic Control of Normal and Pathological Hematopoiesis,Centre de Recherche en Cancérologie de Marseille, 4Unité de Dynamique des Interactions Membranaires Normales et Pathologiques,CNRS UMR5235/DAA/cc107/Université Montpellier II, 5Plateforme BioEmergences IBiSA FBI,CNRS-NED, Institut de Neurobiologie Alfred Fessard
Chapters
- 00:05Title
- 01:21Poly-lysine Coated Dishes
- 02:04Agarose Coated Dishes
- 02:38Injection Mix and Loading Injection Needles
- 03:40Sample Collection
- 04:38Oocyte Injection
- 06:39Fertilization, Selection, and Embryo Culture
- 07:55Results: Expression of Fluorescent Proteins After mRNA Injection
- 08:56Conclusion
We report here the robust and efficient expression of fluorescent proteins after mRNA injection into unfertilized oocytes of Branchiostoma lanceolatum. The development of the microinjection technique in this basal chordate will pave the way for far-reaching technical innovations in this emerging model system, including in vivo imaging and gene-specific manipulations.
