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方法来评估肌肉的亚细胞中<em>温度。线虫</em
JoVE Journal
Biology
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JoVE Journal Biology
Methods to Assess Subcellular Compartments of Muscle in C. elegans
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方法来评估肌肉的亚细胞中<em>温度。线虫</em

DOI:
10.3791/52043-v

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10:13 min

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November 13, 2014

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, , ,
1MRC/ARUK Centre for Musculoskeletal Ageing Research,University of Nottingham

Chapters

  • 00:05Title
  • 01:50Synchronization of L1 Larval Animals Using Gravity Flotation
  • 03:08Movement Assays
  • 03:59Imaging of Mitochondrial Networks and Sarcomeres in Body Wall Muscle and Assessing Membrane Potential
  • 06:13Assessment of Mucle Protein Degradation
  • 07:57Results: The Effect of Muscle Disprution in C. elegans
  • 09:37Conclusion

Summary

Automatic Translation

Automatic Translation

骨骼肌的运动是必不可少的，是机构的主要蛋白质商店。 秀丽隐杆线虫中的肌肉健康测量进行了描述。有意改变肌肉的结构和功能使用本地化的GFP和阳离子染料进行评估。

Tags

Keywords: MuscleC. ElegansMitochondriaSarcomeresGFPCationic DyesMuscle FunctionMuscle StructureMuscle HomeostasisMovement AssaysGenomic RegulationIn Vivo

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