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Use of MALDI-TOF Mass Spectrometry and a Custom Database to Characterize Bacteria Indigenous to a Unique Cave Environment (Kartchner Caverns, AZ, USA)
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Use of MALDI-TOF Mass Spectrometry and a Custom Database to Characterize Bacteria Indigenous to a Unique Cave Environment (Kartchner Caverns, AZ, USA)

Use of MALDI-TOF Mass Spectrometry and a Custom Database to Characterize Bacteria Indigenous to a Unique Cave Environment (Kartchner Caverns, AZ, USA)

DOI:
10.3791/52064-v

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11:09 min

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January 02, 2015

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, , ,
1School of Mathematical and Natural Sciences,Arizona State University, 2Applied Maths NV

Chapters

  • 00:05Title
  • 01:46Deposition on the MALDI Target
  • 02:38Mass Spectra Acquisition Using FlexControl Software
  • 03:56Database Construction Using Bionumerics Software
  • 06:41Mass Spectrum Data Analysis
  • 08:24Bacteria Identification with a Custom Database
  • 09:40Results: Similarity-coefficient-based Method Can Correctly Identify Bacteria to the Species Level
  • 10:33Conclusion

Summary

Automatic Translation

Automatic Translation

This work details procedures for rapid identification of bacteria using MALDI-TOF MS. The identification procedures include spectrum acquisition, database construction, and follow up analyses. Two identification methods, similarity coefficient-based and biomarker-based methods, are presented.

Tags

Keywords: MALDI-TOFMass SpectrometryBacteriaIdentificationKartchner Caverns16S RDNABioNumericsCluster AnalysisPeak MatchingStatistical AnalysisSimilarity CoefficientBiomarker

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