Use of MALDI-TOF Mass Spectrometry and a Custom Database to Characterize Bacteria Indigenous to a Unique Cave Environment (Kartchner Caverns, AZ, USA)

Lin Zhang; Katleen Vranckx; Koen Janssens; Todd R. Sandrin

doi:10.3791/52064

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Use of MALDI-TOF Mass Spectrometry and a Custom Database to Characterize Bacteria Indigenous to a Unique Cave Environment (Kartchner Caverns, AZ, USA)

JoVE Journal
Biology

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JoVE Journal Biology

Use of MALDI-TOF Mass Spectrometry and a Custom Database to Characterize Bacteria Indigenous to a Unique Cave Environment (Kartchner Caverns, AZ, USA)

13,981 Views

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11:09 min

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January 02, 2015

DOI:

10.3791/52064-v

DOI:

10.3791/52064-v

•

11:09 min

January 02, 2015

•

13966 Views

Lin Zhang¹, Katleen Vranckx², Koen Janssens², Todd R. Sandrin¹

¹School of Mathematical and Natural Sciences,Arizona State University ²Applied Maths NV

Summary

This work details procedures for rapid identification of bacteria using MALDI-TOF MS. The identification procedures include spectrum acquisition, database construction, and follow up analyses. Two identification methods, similarity coefficient-based and biomarker-based methods, are presented.