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Slice It Hot: Acute Adult Brain Slicing in Physiological Temperature
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JoVE Journal Neuroscience
Slice It Hot: Acute Adult Brain Slicing in Physiological Temperature

Slice It Hot: Acute Adult Brain Slicing in Physiological Temperature

DOI:
10.3791/52068-v

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08:46 min

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October 30, 2014

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, ,
1Department of Neurobiology,Hebrew University of Jerusalem

Chapters

  • 00:05Title
  • 01:17Setting Up for the Slicing Procedure
  • 02:53Dissecting the Brain
  • 04:52Brain Slicing
  • 07:09Results: Patch-clamp Recordings from Cerebellar Neurons
  • 08:25Conclusion

Summary

Automatic Translation

Automatic Translation

In this paper we show a method for preparing acute brain slices in physiological temperature, using a conventional physiological solution without special modifications for the cutting (such as adding sucrose) and without intracardial perfusion of the animal before slice preparation.

Tags

Acute Brain SlicingPhysiological TemperatureElectrophysiological Patch-clampMyelinated Brain StructuresMature AnimalsNeural HealthTemperature-related ArtifactsSimplified ProcedurePreparation TimeAdult MiceYounger MiceRatsHorizontal Cerebellar SlicesPosterior Brain Areas

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