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Soil Sampling and Isolation of Entomopathogenic Nematodes (Steinernematidae, Heterorhabditidae)
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JoVE Journal Environment
Soil Sampling and Isolation of Entomopathogenic Nematodes (Steinernematidae, Heterorhabditidae)

Soil Sampling and Isolation of Entomopathogenic Nematodes (Steinernematidae, Heterorhabditidae)

DOI:
10.3791/52083-v

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07:45 min

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July 11, 2014

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, ,
1Department of Entomology,University of Arizona

Chapters

  • 00:05Title
  • 01:36Random Soil Sampling
  • 03:12Baiting Nematodes with Insects
  • 04:24Recovering Nematodes from Insects
  • 06:19EPN Collection Using G. mellonella Bait
  • 07:02Conclusion

Summary

Automatic Translation

Automatic Translation

Entomopathogenic nematodes are soil-inhabiting roundworms that parasitize a wide range of insects. We demonstrate sampling methods used for the isolation of these nematodes from the soil using two techniques: the insect baiting and the modified White trap, for the recovery of nematodes from soil samples and infected insect cadavers, respectively.

Tags

Keywords: Entomopathogenic NematodesSteinernematidaeHeterorhabditidaeSoil SamplingInsect-baiting TechniqueWhite Trap TechniqueLaboratory CultureBioassaysModel Organisms

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