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JoVE Journal
Developmental Biology

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Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis
 

Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis

Article DOI: 10.3791/52087
January 12th, 2015

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Summary January 12th, 2015

To analyse cardiac gene expression profiles during zebrafish heart development, total RNA has to be extracted from isolated hearts. Here, we present a protocol for collecting functional/beating hearts by rapid manual dissection from zebrafish embryos to obtain cardiac-specific mRNA.

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