/
/
Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:27Dissecting Zebrafish Embryonic Hearts
- 04:25Isolating mRNA from Dissected Hearts
- 07:44Results: Transcriptional Analysis of Dissected Embryonic Zebrafish Hearts
- 09:38Conclusion
To analyse cardiac gene expression profiles during zebrafish heart development, total RNA has to be extracted from isolated hearts. Here, we present a protocol for collecting functional/beating hearts by rapid manual dissection from zebrafish embryos to obtain cardiac-specific mRNA.
