Peptide array screening is a high throughput assay for identifying protein-protein interaction sites. This allows mapping multiple interactions of a target protein and can serve as a method for identifying sites for inhibitors that target a protein. Here we describe a protocol for screening and analyzing peptide arrays.
Amartely, H., Iosub-Amir, A., Friedler, A. Identifying Protein-protein Interaction Sites Using Peptide Arrays. J. Vis. Exp. (93), e52097, doi:10.3791/52097 (2014).