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In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection
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Immunology and Infection
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JoVE Journal Immunology and Infection
In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection

In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection

DOI:
10.3791/52121-v

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10:39 min

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November 27, 2014

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, , , ,
1Department of Microbiology & Immunology,The University of Texas Medical Branch, 2Department of Pathology,The University of Texas Medical Branch, 3Center for Biodefense and Emerging Infectious Diseases, Sealy Center for Vaccine Development,The University of Texas Medical Branch

Chapters

  • 00:05Title
  • 01:40Flow Cytometry Analysis In Vitro OTII CD4+ T Cell Priming by DC from WNV-infected Mice
  • 05:00Intracellular Cytokine Staining
  • 07:13Results: DC Function and T Cell Responses at an Early Stage of WNV NS4B–P38G Infection
  • 09:45Conclusion

Summary

Automatic Translation

Automatic Translation

Two flow cytometry-based methods – an in vitro T cell priming assay and intracellular cytokine staining were utilized to measure antigen presenting capacity of dendritic cells and antigen-specific T cell responses to a West Nile virus mutant infection in mice.

Tags

Keywords: West Nile VirusWNVNS4B-P38G MutantMyd88T Cell ResponseDendritic CellsIn Vitro T Cell Priming AssayIntracellular Cytokine StainingCFSEOTII Transgenic MiceMicrocentrifuge Tube SystemBiosafety Level 3

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