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December 30, 2014
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Here, we present a protocol that allows the investigator to assess leukocyte recruitment dynamics ex vivo by connecting a chamber coated with endothelial-derived adhesion molecules to the circulatory system of a mouse. This method offers significant advantages since it allows for leukocyte assessment under relative biological conditions.
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Cite this Article
Mulki, L., Sweigard, J. H., Connor, K. M. Assessing Leukocyte-endothelial Interactions Under Flow Conditions in an Ex Vivo Autoperfused Microflow Chamber Assay. J. Vis. Exp. (94), e52130, doi:10.3791/52130 (2014).
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