Ex Vivo Microflow Chamber
Ex Vivo Microflow Chamber - Related Videos
09:01
Assessing Leukocyte-endothelial Interactions Under Flow Conditions in an Ex Vivo Autoperfused Microflow Chamber Assay
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Cited by 2 •
2014
Here, we present a protocol that allows the investigator to assess leukocyte recruitment dynamics ex vivo by connecting a chamber coated with endothelial-derived adhesion molecules to the circulatory system of a mouse. This method offers significant advantages since it allows for leukocyte assessment under relative biological conditions.
09:55
Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber
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Cited by 17 •
2016
This is a guideline for constructing in vivo vascularized tissue using a microsurgical arteriovenous loop or a flow-through pedicle configuration inside a tissue engineering chamber. The vascularized tissues generated can be employed for organ regeneration and replacement of tissue defects, as well as for drug testing and disease modeling.
08:41
Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow
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Cited by 8 •
2016
The protocol describes a novel murine femur window chamber model that can be used to track movement of cells in the femoral bone marrow in vivo. Intravital multiphoton fluorescence microscopy is used to image three components of the femoral bone marrow (vasculature, collagen matrix, and neutrophils) over time.
03:33
Grafting Pancreatic Islets: A Method to Implant Pancreatic Islets into the Anterior Chamber of the Mouse Eye for In Vivo Imaging
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2023
This video describes the protocol for grafting human pancreatic islets into the anterior chamber of the mouse’s eye to monitor revascularization of human islet grafts in vivo. It also helps to understand the contribution of recipient versus donor cells in promoting the encapsulation and vascularization of the graft.
05:27
In Vivo Imaging of Liver Spheroids Engrafted in the Anterior Chamber of the Mouse Eye
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2024
Here, we describe a platform that allows noninvasive in vivo imaging of liver spheroids engrafted in the anterior chamber of the mouse eye. The workflow spans from generating spheroids from primary liver cells to transplantation into the mouse eye and in vivo imaging at cellular resolution by confocal microscopy.
FAQs
Flow rate is a key experimental variable because it sets how quickly culture medium moves through the channel and contributes to the shear stress experienced by the sample. By regulating this rate, investigators can expose isolated tissue or cells to defined mechanical conditions and then relate changes in behavior or barrier function to the imposed flow rather than to an uncontrolled environment.
A pump or pressure gradient supplies the driving force for medium movement through the microscale channel. Controlling this driving condition allows researchers to adjust perfusion and associated shear stress systematically. That control is essential for mechanobiology experiments, where the goal is to examine how cells or tissues respond to fluid forces under defined conditions rather than under variable or poorly characterized flow.
Compared with static culture, the chamber adds a controllable dynamic stimulus and permits real-time observation while the sample is exposed to flow. This combination helps distinguish responses associated with fluid movement from those seen under no-flow conditions. It is therefore useful for examining barrier function, cell behavior, and tissue-device interactions in dynamic tissue environments.
A typical workflow begins by placing the isolated tissue, cells, or biological sample in the chamber and supplying culture medium to the microscale channel. The researcher then establishes flow with a pump or pressure gradient, selects the desired perfusion condition, and observes the sample during exposure. Images or other observations can connect the imposed flow with biological responses.
Core setup components include the ex vivo sample, culture medium, a microscale channel, and a mechanism for driving flow, such as a pump or pressure gradient. The chamber also requires an observation approach that supports real-time monitoring. Together, these elements maintain the sample outside the body while allowing researchers to control perfusion and examine changes under defined flow conditions.
Bioengineers can use the platform to evaluate biomaterials, therapeutic candidates, and engineered tissue constructs under controlled flow. It also supports studies of barrier function, cell behavior, mechanobiology, and tissue-device interactions. Because the sample remains outside the body, researchers can vary perfusion conditions while directly observing biological responses in a controlled experimental setting.