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Detection of In Situ Protein-protein Complexes at the Drosophila Larval Neuromuscular Junction Using Proximity Ligation Assay
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Chapters
- 00:05Title
- 02:09Dissection
- 04:25Fixation, Permeabilization, and Immunochemistry
- 06:12Proximity Ligation Assay and Mounting
- 08:06Results: Detection of Endogenous Protein-protein Complexes at the Drosophila Larval Neuromuscular Junction
- 09:51Conclusion
This protocol demonstrates how Proximity Ligation Assay can be used to detect in situ protein-protein interactions at the Drosophila larval neuromuscular junction. With this technique, Discs large and Hu-li tai shao are shown to form a complex at the postsynaptic region, an association previously identified through co-immunoprecipitation.
