Detection of <em>In Situ</em> Protein-protein Complexes at the <em>Drosophila</em> Larval Neuromuscular Junction Using Proximity Ligation Assay

Simon Wang; SooHyun Yoo; Hae-yoon Kim; Mannan Wang; Clare Zheng; Wade Parkhouse; Charles Krieger; Nicholas Harden

doi:10.3791/52139

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Detection of In Situ Protein-protein Complexes at the Drosophila Larval Neuromuscular Junction Using Proximity Ligation Assay

Detection of <em>In Situ</em> Protein-protein Complexes at the <em>Drosophila</em> Larval Neuromuscular Junction Using Proximity Ligation Assay

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Detection of In Situ Protein-protein Complexes at the Drosophila Larval Neuromuscular Junction Using Proximity Ligation Assay

Detection of In Situ Protein-protein Complexes at the Drosophila Larval Neuromuscular Junction Using Proximity Ligation Assay

13,065 Views

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10:31 min

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January 20, 2015

DOI:

10.3791/52139-v

DOI:

10.3791/52139-v

•

10:31 min

January 20, 2015

•

9 Views

Simon Wang¹, SooHyun Yoo¹, Hae-yoon Kim¹, Mannan Wang², Clare Zheng², Wade Parkhouse², Charles Krieger², Nicholas Harden¹

¹Department of Molecular Biology and Biochemistry,Simon Fraser University, ²Department of Biomedical Physiology and Kinesiology,Simon Fraser University

Summary

This protocol demonstrates how Proximity Ligation Assay can be used to detect in situ protein-protein interactions at the Drosophila larval neuromuscular junction. With this technique, Discs large and Hu-li tai shao are shown to form a complex at the postsynaptic region, an association previously identified through co-immunoprecipitation.