Journal
/
Neuroscience
/
The Olfactory System as a Model to Study Axonal Growth Patterns and Morphology In Vivo
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
The Olfactory System as a Model to Study Axonal Growth Patterns and Morphology In Vivo

The Olfactory System as a Model to Study Axonal Growth Patterns and Morphology In Vivo

DOI:
10.3791/52143-v

•

08:29 min

•

October 30, 2014

•

,
1Institute of Neurophysiology and Cellular Biophysics and Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB),University of Göttingen

Chapters

  • 00:05Title
  • 01:20Preparation of Instruments and Pipette Fabrication
  • 02:43Preparation of Electroporation Solution
  • 03:32Electroporation of Fluorophore-coupled Dextrans
  • 05:27Mounting Animals for In Vivo Visualization of Cells and Axonal Processes
  • 06:44Results: Visualizing Olfactory Axon Projections in the Excised Olfactory Bulb
  • 08:05Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a protocol for in vivo labeling of olfactory sensory neurons by electroporation and subsequent confocal laser-scanning or multiphoton microscopy to visualize neuronal morphology and its development over time.

Tags

Keywords: Olfactory SystemAxonal GrowthAxonal GuidanceNeuron MorphologyIn Vivo LabelingElectroporationFluorescent LabelingLive ImagingXenopus LaevisOlfactory EpitheliumOlfactory BulbNeural Circuit FormationSynapse Formation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/52143?article_type=v

Electrophysiological Measurements from a Moth Olfactory System
check_url/52143?article_type=v

A Caenorhabditis elegans Model System for Amylopathy Study
check_url/52143?article_type=v

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration
check_url/52143?article_type=v

Ex Vivo Preparations of the Intact Vomeronasal Organ and Accessory Olfactory Bulb
check_url/52143?article_type=v

Facial Nerve Surgery in the Rat Model to Study Axonal Inhibition and Regeneration
check_url/52143?article_type=v

Generation of 3-D Collagen-based Hydrogels to Analyze Axonal Growth and Behavior During Nervous System Development
check_url/52143?article_type=v

Combining Behavior and EEG to Study the Effects of Mindfulness Meditation on Episodic Memory
check_url/52143?article_type=v

Coculture of Axotomized Rat Retinal Ganglion Neurons with Olfactory Ensheathing Glia, as an In Vitro Model of Adult Axonal Regeneration
check_url/52143?article_type=v

Utilizing In Vivo Postnatal Electroporation to Study Cerebellar Granule Neuron Morphology and Synapse Development
check_url/52143?article_type=v

Optimizing the Setup and Conditions for Ex Vivo Electroretinogram to Study Retina Function in Small and Large Eyes

Read Article