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Neuroscience

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JoVE Journal Neuroscience
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The Olfactory System as a Model to Study Axonal Growth Patterns and Morphology In Vivo

Article DOI: 10.3791/52143 • 08:29 min • October 30th, 2014
October 30th, 2014 •
1, 1
1Institute of Neurophysiology and Cellular Biophysics and Center for Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB), University of Göttingen

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Summary
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We describe a protocol for in vivo labeling of olfactory sensory neurons by electroporation and subsequent confocal laser-scanning or multiphoton microscopy to visualize neuronal morphology and its development over time.

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Olfactory System Axonal Growth Patterns Axonal Morphology In Vivo Olfactory Stem Cells Sensory Neurons Olfactory Bulb Circuitry Integration Axonal Wiring Guidance Synapse Formation In Vivo Labeling Electroporation Technique Fluorophore-coupled Dextrans Macromolecules Delivery Confocal Laser-scanning Microscopy Multiphoton Microscopy Morphological Changes Time Lapse Imaging Experiments
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