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Derivation and Characterization of a Transgene-free Human Induced Pluripotent Stem Cell Line and Conversion into Defined Clinical-grade Conditions
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Derivation and Characterization of a Transgene-free Human Induced Pluripotent Stem Cell Line and Conversion into Defined Clinical-grade Conditions

Derivation and Characterization of a Transgene-free Human Induced Pluripotent Stem Cell Line and Conversion into Defined Clinical-grade Conditions

DOI:
10.3791/52158-v

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10:48 min

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November 26, 2014

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, ,
1Department of Molecular and Medical Pharmacology,University of California, Los Angeles (UCLA), 2Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research,University of California, Los Angeles (UCLA)

Chapters

  • 00:05Title
  • 01:49Reprogramming Adult Human Dermal Fibroblasts with STEMCCA
  • 04:55Vector Integration Site Analysis and STEMCCA Excision
  • 07:08Conversion into GMP-grade Conditions
  • 08:50Results: Reprogramming hiPSCs Using the STEMCCA Lentiviral Approach
  • 09:59Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a protocol for deriving lentiviral-based reprogrammed and characterized factor-free human induced pluripotent stem cells and conversion into putative clinical-grade conditions.

Tags

Keyword Extraction: Human Induced Pluripotent Stem CellsHiPSCsLentiviral-based ReprogrammingTransgene-freeClinical-grade ConditionsGene ExpressionQuantitative PCRGMP-grade HiPSCsImmunogenicity RiskNon-human AntigensFactor-free Characterized HiPSC-based DerivativesHuman Therapeutic Applications

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