We describe a protocol for deriving lentiviral-based reprogrammed and characterized factor-free human induced pluripotent stem cells and conversion into putative clinical-grade conditions.
Awe, J. P., Vega-Crespo, A., Byrne, J. A. Derivation and Characterization of a Transgene-free Human Induced Pluripotent Stem Cell Line and Conversion into Defined Clinical-grade Conditions. J. Vis. Exp. (93), e52158, doi:10.3791/52158 (2014).