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Production and Use of Lentivirus to Selectively Transduce Primary Oligodendrocyte Precursor Cells for In Vitro Myelination Assays
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Developmental Biology
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JoVE Journal Developmental Biology
Production and Use of Lentivirus to Selectively Transduce Primary Oligodendrocyte Precursor Cells for In Vitro Myelination Assays

Production and Use of Lentivirus to Selectively Transduce Primary Oligodendrocyte Precursor Cells for In Vitro Myelination Assays

DOI:
10.3791/52179-v

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10:00 min

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January 12, 2015

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, , , ,
1Department of Anatomy and Neuroscience,The University of Melbourne, 2The Florey Institute of Neuroscience and Mental Health Research,The University of Melbourne

Chapters

  • 00:05Title
  • 01:242K7 Lentivirus Production
  • 05:07Transducing OPCs and Seeding for Co-culture
  • 08:07Results: Transformed OPCs Differentiating on DRGs
  • 09:32Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present protocols that offer a flexible and strategic foundation for virally manipulating oligodendrocyte precursor cells to overexpress proteins of interest in order to specifically interrogate their role in oligodendrocytes via the in vitro model of central nervous system myelination.

Tags

Keywords: LentivirusPrimary Oligodendrocyte Precursor CellsIn Vitro Myelination AssayDorsal Root Ganglion NeuronsOligodendroglial ProteinsMyelinationCentral Nervous SystemProtein OverexpressionSelective Transduction

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