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Floral-Dip Transformation of Flax (Linum usitatissimum) to Generate Transgenic Progenies with a High Transformation Rate
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Biology
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JoVE Journal Biology
Floral-Dip Transformation of Flax (Linum usitatissimum) to Generate Transgenic Progenies with a High Transformation Rate

Floral-Dip Transformation of Flax (Linum usitatissimum) to Generate Transgenic Progenies with a High Transformation Rate

DOI:
10.3791/52189-v

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08:49 min

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December 19, 2014

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1Department of Biology,Case Western Reserve University

Chapters

  • 00:05Title
  • 01:25Growing the Plants
  • 02:39Floral-Dipping
  • 05:22Selecting Transformants by PCR
  • 07:12Typical Direct PCR Results
  • 08:05Conclusion

Summary

Automatic Translation

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Here, we present a protocol to transform flax using Agrobacterium-mediated plant transformation via floral-dip. This protocol is simple to perform and inexpensive, yet yields a higher transformation rate than the current available methods for flax transformation.

Tags

Keywords: Floral-dip TransformationLinum UsitatissimumAgrobacterium-mediated TransformationBinary Vector PlasmidLinum Insertion Sequence (LIS-1)High Transformation RateT1 SeedsDirect PCRPositive Transformants

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