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An Ex vivo Model of an Oligodendrocyte-directed T-Cell Attack in Acute Brain Slices
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Chapters
- 00:05Title
- 01:30Preparations and Activation of OVA-specific CD8+ T-cells (OT-I)
- 03:34Preparaing of Acute Brain Slices and Co-culture with OT-I T-cells
- 05:25Results: Apoptosis after OD-directed CD8+ T-cell Incubation
- 06:02Conclusion
To address mechanisms of demyelination and neuronal apoptosis in cortical lesions of inflammatory demyelinating disorders, different animal models are used. We here describe an ex vivo approach by using oligodendrocyte-specific CD8+ T-cells on brain slices, resulting in oligodendroglial and neuronal death. Potential applications and limitations of the model are discussed.
