This video describes dissection, tissue processing, sectioning, and fluorescence-based TUNEL labeling of mouse skeletal muscle. It also describes a method for semi-automated analysis of TUNEL labeling.
Fayzullina, S., Martin, L. J. Detection and Analysis of DNA Damage in Mouse Skeletal Muscle In Situ Using the TUNEL Method. J. Vis. Exp. (94), e52211, doi:10.3791/52211 (2014).