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Detection and Analysis of DNA Damage in Mouse Skeletal Muscle In Situ Using the TUNEL Method
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Detection and Analysis of DNA Damage in Mouse Skeletal Muscle In Situ Using the TUNEL Method

Detection and Analysis of DNA Damage in Mouse Skeletal Muscle In Situ Using the TUNEL Method

DOI:
10.3791/52211-v

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09:54 min

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December 16, 2014

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1Division of Neuropathology, Department of Pathology, Pathobiology Graduate Program,Johns Hopkins School of Medicine

Chapters

  • 00:05Title
  • 01:01Fixing, Embedding and Cryosectioning Neonatal Hindlimb Tissue
  • 02:34TUNEL Assay and Fluorescent Labeling
  • 06:02Imaging and Image Analysis
  • 08:01Results: Measuring Apoptosis in a Mouse Model of Spinal Muscular Atrophy
  • 09:34Conclusion

Summary

Automatic Translation

Automatic Translation

This video describes dissection, tissue processing, sectioning, and fluorescence-based TUNEL labeling of mouse skeletal muscle. It also describes a method for semi-automated analysis of TUNEL labeling.

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Keywords: TUNELDNA DamageMouseSkeletal MuscleFluorescenceApoptosisQuantitation

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