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Biology

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Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains
 

Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains

Article DOI: 10.3791/52240 12:21 min
December 13th, 2014

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Summary December 13th, 2014

The steady state level of specific mRNAs is determined by the rate of synthesis and decay of the mRNA. Genome-wide mRNA degradation rates or the decay rates of specific mRNAs can be measured by determining mRNA half-lives. This protocol focuses on measurement of mRNA decay rates in Saccharomyces cerevisiae.

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