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Neuroscience

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Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types
 

Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types

Article doi: 10.3791/52241
December 18th, 2014

Summary December 18th, 2014

We provide a detailed description of a protocol for flow cytometric analysis of surface antigens and/or intracellular antigens in neural cell types. Critical aspects of experimental planning, step-by-step methodological procedures, and fundamental principles of flow cytometry are explained in order to enable neurobiologists to exploit this powerful technology.

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