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JoVE Journal Neuroscience
Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types
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Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types

Article DOI: 10.3791/52241 • 13:05 min
December 18th, 2014 •
1, 1,2, 1,3, 1, 1,4
1Emmy Noether-Group for Stem Cell Biology, Department of Molecular Embryology, Institute of Anatomy and Cell Biology, University of Freiburg, 2Spemann Graduate School of Biology and Medicine and Faculty of Biology, University of Freiburg, 3School of Life Sciences, Keele University, 4Center for Biological Signaling Studies (BIOSS), University of Freiburg

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December 18th, 2014

We provide a detailed description of a protocol for flow cytometric analysis of surface antigens and/or intracellular antigens in neural cell types. Critical aspects of experimental planning, step-by-step methodological procedures, and fundamental principles of flow cytometry are explained in order to enable neurobiologists to exploit this powerful technology.

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