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Deriving Retinal Pigment Epithelium (RPE) from Induced Pluripotent Stem (iPS) Cells by Different Sizes of Embryoid Bodies
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Developmental Biology
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JoVE Journal Developmental Biology
Deriving Retinal Pigment Epithelium (RPE) from Induced Pluripotent Stem (iPS) Cells by Different Sizes of Embryoid Bodies

Deriving Retinal Pigment Epithelium (RPE) from Induced Pluripotent Stem (iPS) Cells by Different Sizes of Embryoid Bodies

DOI:
10.3791/52262-v

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09:18 min

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February 04, 2015

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, , , ,
1Ocular Trauma,U.S. Army Institute of Surgical Research

Chapters

  • 00:05Title
  • 01:52Induced Pluripotent Stem (iPS) Cell Culture and Passaging
  • 03:39Generation, Plating, and Differentiation of Embryoid Bodies (EBs)
  • 06:28Results: Representative Retinal Pigment Epithelium (RPE)
  • 08:11Conclusion

Summary

Automatic Translation

Automatic Translation

The objective of this report is to describe the protocols to derive the retinal pigment epithelium (RPE) from induced pluripotent stem (iPS) cells using different sizes of embryoid bodies.

Tags

Keywords: Induced Pluripotent Stem CellsRetinal Pigment EpitheliumEmbryoid BodiesDifferentiationMicrowell PlatesImmunocytochemistryRT-PCRFACS Analysis

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