JoVE Journal
Neuroscience
Neuroscience
A subscription to JoVE is required to view this content.
Chapters
Summary
This paper provides a method for investigating neurotransmitter vesicle dynamics in neuroblastoma cells, using a synaptobrevin2-pHluorin construct and Total Internal Reflection Fluorescence Microscopy. The strategy developed for image processing and data analysis is also reported.