This content is Free Access.
Chapters
- 00:05Title
- 01:21Cryo-holder Preparation and Maintenance
- 02:32Grid Preparation
- 03:48Plunge-freezing
- 04:50Transfer the Cryo-grid to the TEM
- 06:15Low-dose Data Collection
- 07:03Results: Visualization of Macromolecules Using Cryo-electron Microscopy
- 08:51Conclusion
This paper describes the cryo-electron microscopy methodology, which is used to obtain high quality microscopic images of macromolecules in their near-native state. The method yields images suitable for further computerized processing using the single particle approach, devised to generate the 3D reconstruction of a macromolecule.
Tags
Keywords: Cryo-electron MicroscopyCryoEMSample PreparationMacromolecular 3D ReconstructionTransmission Electron MicroscopyTEMSingle-particle Image Processing3D Structural DeterminationPurificationVitrificationImage Processing SoftwareStructural DeterminationMacromolecular MachinesPseudo-atomic StructuresAtomic Resolution
