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Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy
JoVE Journal
Medicine
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JoVE Journal Medicine
Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy

Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy

DOI:
10.3791/52350-v

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11:27 min

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February 07, 2015

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, , , , ,
1Department of Internal Medicine, Section on Gerontology and Geriatric Medicine,Wake Forest School of Medicine, 2Seahorse Biosciences

Chapters

  • 00:05Title
  • 01:38Skeletal Muscle Biopsy
  • 03:20Mitochondrial Isolation and Washing
  • 06:35Perform Extracellular Flux (XF) Assays
  • 08:32Results: Respirometric Assessment of Mitochondria
  • 10:11Conclusion

Summary

Automatic Translation

Automatic Translation

Methods for biopsy of Vastus lateralis, preparation of purified mitochondria, and respirometric profiling are described. The use of small muscle volume makes this technique suitable for clinical research applications.

Tags

Keywords: MitochondriaSkeletal MusclePercutaneous Needle BiopsyRespirometryExtracellular Flux AnalysisElectron Transport ChainCOX IVGAPDH

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