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February 07, 2015
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Methods for biopsy of Vastus lateralis, preparation of purified mitochondria, and respirometric profiling are described. The use of small muscle volume makes this technique suitable for clinical research applications.
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Cite this Article
Bharadwaj, M. S., Tyrrell, D. J., Lyles, M. F., Demons, J. L., Rogers, G. W., Molina, A. J. A. Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy. J. Vis. Exp. (96), e52350, doi:10.3791/52350 (2015).
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