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JoVE Journal Neuroscience
Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings
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Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings

Article DOI: 10.3791/52358 • 10:24 min • January 10th, 2015
January 10th, 2015 •
1, 1, 1,2
1Institute of Neuroscience and Medicine (INM-2), Research Centre Jülich, 2Department of Psychiatry, Psychotherapy and Psychosomatics, Medical Faculty, JARA, RWTH Aachen University

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Summary
Automatic Translation

Patch-clamp recordings and simultaneous intracellular biocytin filling of synaptically coupled neurons in acute brain slices allow a correlated analysis of their structural and functional properties. The aim of this protocol is to describe the essential technical steps of electrophysiological recording from neuronal microcircuits and their subsequent morphological analysis.

Tags

Electrophysiology Morphological Characterization Neuronal Microcircuits Acute Brain Slices Patch-clamp Recordings Paired Recordings Intracellular Biocytin Filling Structural Properties Functional Properties Pre- And Postsynaptic Neurons Connectivity Patterns Chemical Synaptic Transmission Electrical Synaptic Transmission Neuron Morphology Synaptic Contacts Dendritic Domain Axonal Domain
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