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Generation of CAR T Cells for Adoptive Therapy in the Context of Glioblastoma Standard of Care
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Immunology and Infection
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JoVE Journal Immunology and Infection
Generation of CAR T Cells for Adoptive Therapy in the Context of Glioblastoma Standard of Care

Generation of CAR T Cells for Adoptive Therapy in the Context of Glioblastoma Standard of Care

DOI:
10.3791/52397-v

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12:55 min

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February 16, 2015

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, , , , ,
1Duke Brain Tumor Immunotherapy Program, Department of Neurosurgery,Duke University, 2Department of Biomedical Engineering,Duke University, 3Department of Pathology,Duke University

Chapters

  • 00:05Title
  • 01:59Administration of Temozolomide and Whole Brain Irradiation to Tumor-bearing Mice (days 0-4)
  • 03:26Cell Transfection, Splenectomy, and T Cell Preparation (day 0)
  • 06:09Transduction (days 1 and 2)
  • 08:10CAR T Cell and Administration to Tumor-bearing Mice (days 3-5)
  • 10:03Results: Antitumor Efficacy of CAR T Cells
  • 11:47Conclusion

Summary

Automatic Translation

Automatic Translation

The lymphodepletive and immunomodulatory effects of chemotherapy and radiation standard of care can be leveraged to enhance the antitumor efficacy of T cell immunotherapy. We outline a method for generating EGFRvIII-specific chimeric antigen receptor (CAR) T cells and administering them in the context of glioblastoma standard of care.

Tags

Keywords: CAR T CellsAdoptive ImmunotherapyGlioblastomaEGFRvIIIStandard Of CareTemozolomideWhole Brain Irradiation

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