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1Department of Medicine, Division of Liver Diseases,Icahn School of Medicine at Mount Sinai, 2Department of Otolaryngology,Icahn School of Medicine at Mount Sinai, 3Division of Nephrology,Columbia University College of Physicians and Surgeons, 4Departments of Medicine, Hematology and Medical Oncology,Icahn School of Medicine at Mount Sinai, 5Bobby R. Alford Department of Otolaryngology – Head and Neck Surgery,Baylor College of Medicine
Chapters
- 00:05Title
- 01:44Preparing the Egg for Inoculation: “Dropping” the CAM and Opening the Egg
- 03:57Inoculation of the CAM with Tumor Cells
- 05:32Harvesting Tumors from the CAM
- 06:24Tumor Cell Dissociation
- 07:20Results: The CAM Supports Growth of Three-Dimensional, Vascularized Tumors
- 07:44Conclusion
The chick chorioallantoic membrane (CAM) is immunodeficient and highly vascularized, making it a natural in vivo model of tumor growth and angiogenesis. In this protocol, we describe a reliable method of growing three-dimensional, vascularized hepatocellular carcinoma (HCC) tumors using the CAM assay.
