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The In Ovo Chick Chorioallantoic Membrane (CAM) Assay as an Efficient Xenograft Model of Hepatocellular Carcinoma

Article DOI: 10.3791/52411-v • 08:17 min • October 9th, 2015
October 9th, 2015 •
1,2, 5, 3, 1, 4, 5
1Department of Medicine, Division of Liver Diseases, Icahn School of Medicine at Mount Sinai, 2Department of Otolaryngology, Icahn School of Medicine at Mount Sinai, 3Division of Nephrology, Columbia University College of Physicians and Surgeons, 4Departments of Medicine, Hematology and Medical Oncology, Icahn School of Medicine at Mount Sinai, 5Bobby R. Alford Department of Otolaryngology - Head and Neck Surgery, Baylor College of Medicine

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Summary
Automatic Translation

The chick chorioallantoic membrane (CAM) is immunodeficient and highly vascularized, making it a natural in vivo model of tumor growth and angiogenesis. In this protocol, we describe a reliable method of growing three-dimensional, vascularized hepatocellular carcinoma (HCC) tumors using the CAM assay.

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In Ovo Chick Chorioallantoic Membrane (CAM) Assay Efficient Xenograft Model Hepatocellular Carcinoma CAM Development Immunodeficient Highly Vascularized Tumor Implantation Extracellular Matrix Proteins Fibronectin Laminin Collagen Integrin Alpha(v)beta3 MMP-2 Tumor Invasion Metastasis Angiogenesis Model In Vivo Xenograft Model System In Vitro Work Animal Cancer Models Tumor Growth Study Anti-tumor Therapies Pro-tumor Molecular Pathways Biologically Relevant System Cost-effective Time-effective CAM Xenograft Model
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