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Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification
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Bioengineering
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JoVE Journal Bioengineering
Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification

Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification

DOI:
10.3791/52423-v

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09:11 min

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February 19, 2015

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,
1Centre for Vascular Research,University of New South Wales, 2School of Medical Sciences,University of New South Wales

Chapters

  • 00:05Title
  • 02:13Quantifying Endothelial Cell De-adhesion from Fibronectin in Response to MPO-mediated Fibronectin Oxidation
  • 06:03Data Analysis and Presentation
  • 07:11Results: Quantification of Endothelial Cell De-adhesion in Response to MPO-mediated Fibronectin Oxidation
  • 08:35Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to continuously quantify cell adhesion and de-adhesion processes with high temporal resolution in a non-invasive manner by cell-substrate impedance and live cell imaging analyses. These approaches reveal the dynamics of cell adhesion/de-adhesion processes triggered by matrix modification and their temporal relationship to adhesion-dependent signaling events.

Tags

Keywords: Cell-matrix AdhesionCell-substrate ImpedanceLive Cell ImagingMatrix ModificationMyeloperoxidaseEndothelial CellsCell MorphologyCell SignalingCell Adhesion DynamicsExtracellular Matrix

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