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JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Macrophage Cholesterol Depletion and Its Effect on the Phagocytosis of Cryptococcus neoformans
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Macrophage Cholesterol Depletion and Its Effect on the Phagocytosis of Cryptococcus neoformans

Article DOI: 10.3791/52432
December 19th, 2014 •
*1, *1, 1, 1
1Department of Molecular Genetics and Microbiology, Stony Brook University
* These authors contributed equally

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December 19th, 2014

In this article, a protocol for infection of macrophages with Cryptococcus neoformans is described. Also, a method for sterol depletion from the macrophages is explained. These protocols provide a guide to study fungal infections in vitro and examine the role of sterols in such infections.

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