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Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy
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Medicine
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JoVE Journal Medicine
Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy

Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy

DOI:
10.3791/52462-v

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08:09 min

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April 06, 2015

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, , , , , , , , , ,
1Department of Diagnostic and Interventional Radiology,University Medical Center, Hamburg, 2Institute of Immunology,University Medical Center, Hamburg, 3University Cancer Center Hamburg,University Medical Center, Hamburg, 4Department of Oncology and Hematology,University Medical Center, Hamburg

Chapters

  • 00:05Title
  • 02:11In Vivo Imaging
  • 03:07Harvesting and Preparation of Tumors
  • 04:19FACS Analysis
  • 06:01Results: Nanobody and mAb Staining of Tumors
  • 07:09Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol outlines the steps required to perform ex vivo validation of in vivo near-infrared fluorescence xenograft imaging experiments in mice using fluorophore labelled nanobodies and conventional antibodies.

Tags

NanobodyAntibodyIn VivoTumor XenograftNIRF ImagingFlow CytometryMicroscopySubcutaneous TumorAntigen-negativeAntigen-positiveAlexaFluor680Non-invasive ImagingEx Vivo Validation

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