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Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy

Article DOI: 10.3791/52462 • 08:09 min • April 6th, 2015
April 6th, 2015 •
*1, *1,2, 1,2, 1,2, 2, 1,2, 2, 3, 4, 1, 2
1Department of Diagnostic and Interventional Radiology, University Medical Center, Hamburg, 2Institute of Immunology, University Medical Center, Hamburg, 3University Cancer Center Hamburg, University Medical Center, Hamburg, 4Department of Oncology and Hematology, University Medical Center, Hamburg
* These authors contributed equally

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Summary
Automatic Translation

This protocol outlines the steps required to perform ex vivo validation of in vivo near-infrared fluorescence xenograft imaging experiments in mice using fluorophore labelled nanobodies and conventional antibodies.

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Validation Nanobody Antibody In Vivo Tumor Xenograft NIRF-imaging Experiments Mice Ex Vivo Flow Cytometry Microscopy Subcutaneous Tumors Antigen-negative Cell Lines Antigen-positive Cells Intravenous Administration Near-infrared Fluorophore Labelled Nanobodies Conventional Antibodies Intraindividual Comparison Small-animal NIRF-Imaging System Sacrificed Mice Parallel Ex Vivo Analyses Quantification Labeling Efficiency Tumor Cells Distribution Antibody Constructs Near-infrared Fluorophore Labelled Probes
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