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Generation of Murine Cardiac Pacemaker Cell Aggregates Based on ES-Cell-Programming in Combination with Myh6-Promoter-Selection
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Developmental Biology
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JoVE Journal Developmental Biology
Generation of Murine Cardiac Pacemaker Cell Aggregates Based on ES-Cell-Programming in Combination with Myh6-Promoter-Selection

Generation of Murine Cardiac Pacemaker Cell Aggregates Based on ES-Cell-Programming in Combination with Myh6-Promoter-Selection

DOI:
10.3791/52465-v

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08:52 min

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February 17, 2015

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, ,
1Reference and Translation Center for Cardiac Stem Cell Therapy,University of Rostock

Chapters

  • 00:05Title
  • 01:19Differentiation Procedure
  • 04:56Selection of Sinus Nodal Cells
  • 07:12Results: Generation of Cardiac Pacemaker Cell Aggregates
  • 08:20Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes how to produce functional sinus nodal tissue from murine pluripotent stem cells (PSC). T-Box3 (TBX3) overexpression plus cardiac Myosin-heavy-chain (Myh6) promoter antibiotic selection leads to highly pure pacemaker cell aggregates. These “Induced-sinoatrial-bodies” (“iSABs”) contain over 80% pacemaker cells, show highly increased beating rates and are able to pace myocardium ex vivo.

Tags

Keywords: Murine Cardiac Pacemaker CellsES-cell-programmingMyh6-promoter-selectionInduced-sinoatrial-bodies (iSABs)Sinus Node Inducer TBX3Pacemaker CellsSpontaneous ContractionNodal Cells

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