Antibiotic Selection
Antibiotic Selection - Related Videos
01:45
Antibiotic Selection
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2026
Researchers use antibiotic resistance genes to identify bacteria that possess a plasmid containing their gene of interest. Antibiotic resistance naturally occurs when a spontaneous DNA mutation creates changes in bacterial genes that eliminate antibiotic activity. Bacteria can share these new resistance genes with their offspring and other bacteria. The overuse and misuse of antibiotics have created a public health crisis, as resistant and multi-resistant bacteria continue to develop.Antibiotic...
02:30
F Plasmid Transfer and Dual Antibiotic Selection in E. coli
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2025
Source: Erdogan, F.,et al., Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation. J. Vis. Exp. (2017)This video demonstrates the procedure for transferring an F plasmid from donor to recipient E. coli cells using a rescue plasmid and selecting transconjugants through dual antibiotic resistance. The process includes mixing bacterial cultures, incubation without shaking, disrupting mating pairs, and plating on selective media to confirm...
10:50
Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection
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Cited by 14 •
2016
We describe a low cost, configurable morbidostat that enables the characterization of antibiotic drug resistance by dynamically adjusting the drug concentration. The device can be integrated with a multiplexed microfluidic platform. The approach can be scaled up for laboratory antibiotic drug resistance studies.
13:39
Antibiotic Susceptibility Testing: Epsilometer Tests to Determine MIC Values of Two Antibiotics and Evaluate Antibiotic Synergy
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2023
Source: Anna Bläckberg1, Rolf Lood1 1 Department of Clinical Sciences Lund, Division of Infection Medicine, Biomedical Center, Lund University, 221 00 Lund Sweden Knowledge of the interactions between antibiotics and bacteria is important in understanding how microbes evolve antibiotic resistance. In 1928, Alexander Fleming discovered penicillin, an antibiotic that exerts its antibacterial function by interfering with cell wall regeneration (1). Other antibiotics with diverse mechanisms of...
10:49
Antibiotic Dereplication Using the Antibiotic Resistance Platform
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Cited by 2 •
2019
We describe a platform that utilizes a library of isogenic antibiotic resistant Escherichia coli for the dereplication of antibiotics. The identity of an antibiotic produced by bacteria or fungi can be deduced by the growth of E. coli expressing its respective resistance gene. This platform is economically effective and time-efficient.
FAQs
The antibiotic creates a growth condition in which the resistance gene becomes functionally important. Cells that express the corresponding protective protein can continue growing, whereas non-resistant cells are inhibited or killed. This difference converts a mixed population into an enriched population of cells carrying the resistance-linked genetic construct.
Linking resistance to the construct makes antibiotic survival an indicator of construct carriage. When cells grow in the antibiotic-containing culture, researchers can recover populations enriched for cells that also carry the associated recombinant DNA. The linkage therefore connects an easily observed growth outcome with the presence of a less directly visible genetic modification.
The resistance gene directs production of a protective protein that allows the cell to withstand the antibiotic condition. Without this protection, susceptible cells cannot continue normally in the culture and may be inhibited or killed. Selection therefore depends on gene expression, not merely on the physical presence of DNA in a cell.
Following plasmid transformation, cells are placed in a culture containing the antibiotic associated with the plasmid's resistance gene. Growth under this condition enriches for cells that retained the plasmid, including recombinant plasmids when the desired DNA is present in the construct. This step simplifies recovery before downstream molecular cloning or analysis.
Researchers can continue culturing engineered cells under the relevant antibiotic condition to favor retention of the resistance-linked genetic construct. Cells lacking the construct are inhibited or killed, while resistant cells remain able to grow. This provides a practical way to maintain modified populations during studies requiring ongoing culture of engineered cells.
Antibiotic selection supports several workflows that depend on recovering or maintaining genetically modified cells. In molecular cloning, it helps identify cells carrying recombinant DNA. The same strategy contributes to studies of gene function and protein production by enriching for engineered cells that contain the relevant genetic construct and can be cultured for further investigation.