Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

Maria Weinert; Tharakeswari Selvakumar; Travis S. Tierney; Kambiz N. Alavian

doi:10.3791/52475

Journal

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Neuroscience

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Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

13,859 Views

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08:45 min

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February 19, 2015

DOI:

10.3791/52475-v

DOI:

10.3791/52475-v

•

08:45 min

February 19, 2015

•

3 Views

Maria Weinert¹, Tharakeswari Selvakumar², Travis S. Tierney², Kambiz N. Alavian^1,3

¹Division of Brain Sciences, Department of Medicine,Imperial College London, ²Department of Neurosurgery,Brigham and Women’s Hospital, Harvard Medical School, ³Department of Internal Medicine, Endocrinology,Yale University School of Medicine

Summary

The causes of degeneration of midbrain dopaminergic neurons during Parkinson’s disease are not fully understood. Cellular culture systems provide an essential tool for study of the neurophysiological properties of these neurons. Here we present an optimized protocol, which can be utilized for in vitro modeling of neurodegeneration.