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Neuroscience

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Conditional Genetic Transsynaptic Tracing in the Embryonic Mouse Brain
 

Conditional Genetic Transsynaptic Tracing in the Embryonic Mouse Brain

Article doi: 10.3791/52487
December 22nd, 2014

Summary December 22nd, 2014

Capitalizing on a binary genetic strategy we provide a detailed protocol for neural circuit tracing in mice that express complementary transsynaptic tracers after Cre-mediated recombination. Because cell-specific tracer production is genetically encoded, our experimental approach is suitable to study the formation and maturation of neural circuitry during murine embryonic brain development at a single cell resolution.

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