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JoVE Journal
Biology

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Targeted DNA Methylation Analysis by Next-generation Sequencing
 

Targeted DNA Methylation Analysis by Next-generation Sequencing

Article DOI: 10.3791/52488
February 24th, 2015

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Summary February 24th, 2015

Bisulfite amplicon sequencing (BSAS) is a method for quantifying cytosine methylation in targeted genomic regions of interest. This method uses bisulfite conversion paired with PCR amplification of target regions prior to next-generation sequencing to produce absolute quantitation of DNA methylation at a base-specific level.

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