Journal
/
Biology
/
Targeted DNA Methylation Analysis by Next-generation Sequencing
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Targeted DNA Methylation Analysis by Next-generation Sequencing

Targeted DNA Methylation Analysis by Next-generation Sequencing

DOI:
10.3791/52488-v

•

08:38 min

•

February 24, 2015

•

, ,
1Department of Physiology,University of Oklahoma College of Medicine, 2Department of Geriatric Medicine,University of Oklahoma College of Medicine

Chapters

  • 00:05Title
  • 01:26Bisulfite Specific PCR Optimization
  • 02:33Next Generation Sequencing (NGS) Library Preparation, Library Quality Control, and Methylation Quantification Data Analysis
  • 06:29Results: Representative Methylation Quantification
  • 08:14Conclusion

Summary

Automatic Translation

Automatic Translation

Bisulfite amplicon sequencing (BSAS) is a method for quantifying cytosine methylation in targeted genomic regions of interest. This method uses bisulfite conversion paired with PCR amplification of target regions prior to next-generation sequencing to produce absolute quantitation of DNA methylation at a base-specific level.

Tags

Keywords: DNA MethylationEpigeneticsNext-generation SequencingBisulfite SequencingBisulfite Amplicon SequencingTargeted AnalysisGene ExpressionCytosine MethylationCpG IslandsQuantitative Analysis

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/52488?article_type=v

Determination of DNA Methylation of Imprinted Genes in Arabidopsis Endosperm
check_url/52488?article_type=v

DNA Methylation: Bisulphite Modification and Analysis
check_url/52488?article_type=v

Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity
check_url/52488?article_type=v

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
check_url/52488?article_type=v

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
check_url/52488?article_type=v

Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites
check_url/52488?article_type=v

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing
check_url/52488?article_type=v

Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing
check_url/52488?article_type=v

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
check_url/52488?article_type=v

A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing

Read Article