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February 24, 2015
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Bisulfite amplicon sequencing (BSAS) is a method for quantifying cytosine methylation in targeted genomic regions of interest. This method uses bisulfite conversion paired with PCR amplification of target regions prior to next-generation sequencing to produce absolute quantitation of DNA methylation at a base-specific level.
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Cite this Article
Masser, D. R., Stanford, D. R., Freeman, W. M. Targeted DNA Methylation Analysis by Next-generation Sequencing. J. Vis. Exp. (96), e52488, doi:10.3791/52488 (2015).
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