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Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis
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1Department of Cell and Developmental Biology,Sackler Faculty of Medicine, Tel Aviv University, 2Division of Allergy and Immunology, Department of Pediatrics,Cincinnati Children’s Hospital Medical Center, University of Cincinnati College of Medicine, 3Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences,Graduate School of Life Sciences, Tohoku University
Chapters
- 00:05Title
- 02:37Transfection of Rat Basophilic Leukemia (RBL) Cells
- 06:06Measuring NPY mRFP Exocytosis
- 07:48Time-lapse Microscopy of Exocytosis
- 10:51Image Analyses
- 13:00Results: Quantifying Mast Cells Secretory Granule (SG) Size and their Exocytosis
- 14:44Conclusion
The goal of the present protocol was to develop a method that will allow functional genomic analyses of mast cell secretion. The protocol is based on quantitative assessment of the release of a fluorescent reporter gene cotrasfected with the gene of interest and real time analyses of the secretory granule's morphology.
