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Journal / Immunology and Infection / Undersøgelse Mast Cell Sekretorisk Granulat; fra…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Undersøgelse Mast Cell Sekretorisk Granulat; fra Biosyntese til exocytose
Zebrafish Animal Model for the Study of Allergic Reactions in Response to Tick Saliva Biomolecules
Journal (Immunology and Infection)
Zebrafish Animal Model for the Study of Allergic Reactions in Response to Tick Saliva Biomolecules
Imaging the Human Immunological Synapse
Journal (Immunology and Infection)
Imaging the Human Immunological Synapse
 
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Undersøgelse Mast Cell Sekretorisk Granulat; fra Biosyntese til exocytose

Article DOI: 10.3791/52505-v • 16:01 min • January 26th, 2015
January 26th, 2015 •
1,2, 3, 2, 1
1Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, 2Division of Allergy and Immunology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, 3Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University

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Målet med denne protokol var at udvikle en metode, der vil gøre det muligt for funktionelle genomiske analyser af mast celle sekretion. Protokollen er baseret på kvantitativ vurdering af udgivelsen af ​​en fluorescerende reporter gen cotrasfected med genet af interesse og tidstro analyser af sekretoriske granula morfologi.

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Immunology mastceller exocytose sekretoriske granuler live cell imaging Rab5 reportergen konfokal billeddannelse transfektion
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