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Journal / Immunology and Infection / Enquête mât cellules sécrétoires granulés; de…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Enquête mât cellules sécrétoires granulés; de biosynthèse d'exocytose
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Enquête mât cellules sécrétoires granulés; de biosynthèse d'exocytose

Article DOI: 10.3791/52505-v • 16:01 min • January 26th, 2015
January 26th, 2015 •
1,2, 3, 2, 1
1Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, 2Division of Allergy and Immunology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, 3Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University

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L'objectif du présent Protocole est de développer une méthode qui permettra aux analyses de génomique fonctionnelle de la sécrétion des mastocytes. Le protocole est basé sur l'évaluation quantitative de la libération d'un gène rapporteur fluorescent cotrasfected avec le gène d'intérêt et des analyses en temps réel de la morphologie du granule sécrétoire.

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Immunologie Numéro 95 mastocytes exocytose granules sécrétoires imagerie des cellules vivantes Rab5 gène rapporteur l'imagerie confocale la transfection
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