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JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Contrast Imaging in Mouse Embryos Using High-frequency Ultrasound
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Contrast Imaging in Mouse Embryos Using High-frequency Ultrasound

Article DOI: 10.3791/52520
March 4th, 2015 •
1,2, 1,2, 1,2,3, 1,2
1Department of Medical Biophysics, University of Toronto, 2Sunnybrook Research Institute, 3Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto

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March 4th, 2015

Here, we present a protocol to inject ultrasound microbubble contrast agents into living, isolated late-gestation stage murine embryos. This method enables the study of perfusion parameters and of vascular molecular markers within the embryo using contrast-enhanced high-frequency ultrasound imaging.

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