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Assessing Transmissible Spongiform Encephalopathy Species Barriers with an In Vitro Prion Protein Conversion Assay
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Assessing Transmissible Spongiform Encephalopathy Species Barriers with an In Vitro Prion Protein Conversion Assay

Assessing Transmissible Spongiform Encephalopathy Species Barriers with an In Vitro Prion Protein Conversion Assay

DOI:
10.3791/52522-v

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11:41 min

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March 10, 2015

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, , , ,
1USGS National Wildlife Health Center, 2Department of Soil Science,University of Wisconsin–Madison, 3Laboratory of Immunology, National Institute of Allergy and Infectious Diseases,National Institutes of Health, 4Merial Veterinary Scholars Program, School of Veterinary Medicine,University of Wisconsin–Madison, 5Department of Neurology,University of British Columbia

Chapters

  • 00:05Title
  • 01:41Preparation of Conversion Efficiency Ratio (CER) Assay Substrate Pairs
  • 05:30Preparation of Transmissible Spongiform Encephalopathy (TSE) Agent Seeds
  • 06:16Conversion Efficiency Ratio (CER) Assay
  • 09:19Results: Conversion of PrPc from Mouse Substrate to PrPres
  • 10:58Conclusion

Summary

Automatic Translation

Automatic Translation

Measuring the barrier to the interspecies transmission of prion diseases is challenging and typically involves animal challenges or biochemical assays. Here, we present an in vitro prion protein conversion assay with the ability to predict species barriers.

Tags

Keywords: PrionTransmissible Spongiform EncephalopathySpecies BarrierIn Vitro AssayConversion Efficiency RatioCellular Prion ProteinProteinase KChronic Wasting DiseaseBobcatWhite-tailed Deer

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